Informatics Publishing Limited

H. Mahabal Rashmi ¹, G. Deshchougule Sayali ¹, M. Suryawanshi Vishnu ¹, R. Chabukswar Anuruddha ¹, P. Choudhari Vishnu ¹, S. Kuchekar Bhanudas ¹

Affiliations
1 Pharmaceutical Analysis and Quality Assurance Department, MAEER's Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune, 411038, MS, IN

Abstract

Two simple, rapid, accurate and economic spectrophotometric methods have been developed for the determination of Atenolol (ATE)&Lercanidipine Hydrochloride (LER) in combined dosage form. The first method is Area under curve (AUC) and second is Absorption Corrected method. The wavelength ranges 260-264&280-285 were selected to determine Atenolol (ATE)&Lercanidipine (LER), respectively in combined formulation by AUC method. The second method was absorption corrected method in which both the drugs exhibited strong absorbance at 270.33 nm. However, LER exhibited strong absorbance at 353.93, at which ATE showed zero absorbance. Hence, 353.93 nm was selected as one wavelength for determination of LER without interference from ATE. Quantitative estimation of ATE was carried out by subtracting the absorption due to LER at 270.33 nm using experimentally calculated absorption factor. Beer's law was obeyed in the concentration range of 25-300 μg/ml for ATE and 5-60 μg/ml for LER for both the methods. The results of analysis have been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods which were carried out by following ICH guidelines.

Keywords

Lercanidipine Hydrochloride, Atenolol, Area under Curve, Absorption Corrected.

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S. Patil Shamkant ¹, N. Dhabale Pandurang ¹, S. Kuchekar Bhanudas ¹

Affiliations
1 Govt. College of Pharmacy, Vidyanagar, Karad-415124, Maharashtra, IN

Abstract

Three simple, precise and economical UV methods have been developed for the estimation of Esomeprazole in bulk and pharmaceutical formulations. Esomeprazole has the absorbance maxima at 303nm (Method A), and in the first order derivative spectra, showed zero crossing at 303nm, with a sharp peak at 292nm when n=1 (Method B), Method C applied was Area Under Curve (AUC) for analysis of Esomeprazole in the wavelength range of 294- 310nm. Drug followed the Beer’s Lamberts range of 5-40 μg/ml for the Method A, B C. Results of analysis were validated statistically and by recovery studies and were found to be satisfactory.

Keywords

Esomeprazole, UV Spectrophotometry, Derivative Spectroscopy, Area Under Curve.

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